Journal: Oxidative Medicine and Cellular Longevity
Article Title: Testosterone Upregulates the Expression of Mitochondrial ND1 and ND4 and Alleviates the Oxidative Damage to the Nigrostriatal Dopaminergic System in Orchiectomized Rats
doi: 10.1155/2017/1202459
Figure Lengend Snippet: The effects of TP supplements on ND1 and ND4 in the SN of GDX rats. (a-b) ND1 or ND4 mRNA was detected by qPCR. (c–f) ND1 or ND4 protein was measured by Western blot. n = 8 per group, expressed as mean ± SD. ∗ P < 0.01.
Article Snippet: The membrane was rinsed in three changes of TBST and then incubated overnight with mouse anti-TH monoclonal antibody (1 : 10,000; T2928, Sigma), rabbit anti-DAT polyclonal antibody (1 : 4000; AB2231, Millipore), rabbit anti-ND1 monoclonal antibody (1 : 2000; ab181848, Abcam), or rabbit anti-ND4 monoclonal antibody (1 : 200; HPA053928, Sigma) at 4°C according to the experiment purposes.
Techniques: Western Blot